Flow cytometry may be used to analyse cell cycle in live eGFP Gli2 transduced human keratinocytes, NTERTs cells by loading cells with Hoechst 33342 at 10 ug/ml for 45 mins at 37C. The determination of ...

The Flow Cytometry and Cell Separation Facility provides advanced cell sorting and cell analysis techniques including immunofluorescence detection of 18 colors, cell-cycle distribution, apoptosis ...

With the dye, cells may be analyzed by fluorescence microscopy, flow cytometry and laser-scanning cytometry. The ability to identify cell cycle position through DNA content analysis is fundamental ...

Flow cytometry has previously been employed not only to measure LC3B but combine this with cell cycle analysis to demonstrate cell cycle arrest by rapamycin and various nutrients. The current methods ...

Modern flow cytometry dates back to the early 1970s when a team at Stanford University, led by Leonard Herzenberg, merged antibodies tagged with fluorescent labels with cell sorting and ...