The score is a value from 1 to 10, similar to other RNA quality scores. With the Qubit RNA IQ Assay, a small number indicates that the sample is comprised of mainly small RNA and a larger number ...

RNA, and protein using the highly sensitive Qubit quantitation assays. The concentration of the target molecule in the sample is reported by a fluorescent dye that emits a signal only when bound ...

The assay was performed as per the manufacturer’s guidelines. The isolated RNA was quantified using RNA HS Kit (Qubit, Cat No.: Q32855). 10–100 ng of purified total RNA was used as a starting material ...

Each RNA sample was quantified using the Qubit RNA High Sensitivity kit (Invitrogen) and Bioanalyzer RNA Pico kit ... All samples were initially diluted 1:100 (in addition to the 1:10 assay buffer ...

RNA was extracted using ‘miRNeasy’ Kit, (Qiagen). RNA integrity was assessed on Qubit RNA HS Assay Kit (Invitrogen). Small RNAs were converted to complementary DNA libraries using the NEXTFLEX Small ...

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The quantity and quality of the extracted RNA were determined by Invitrogen Qubit Flex Fluorometer ... First Strand cDNA Synthesis Kit (TaKaRa, Dalian, China). The CDSs of VvCYP76F14 were cloned using ...

Complementary whole-blood RNA ... Prep Kits for Illumina (NEB) and NEBNext Globin & rRNA Depletion Kits according to the manufacturer’s instructions. The fragment sizes were determined with a Fragment ...

HD mice and human samples reveal m 6 A methylation in the same intronic DRACH motif located in intron 1 of huntingtin RNA. Pharmacological inhibition and siRNA knockdown of METTL3, as well as targeted ...