hPSCs were maintained in a standard feeder-free culture system using mTeSR1 medium … さらに表示

Three different kinds of substrate were used in this study: glass coverslip coated with 1% Geltrex solution for 1 h at room temperature, Geltrex beds of different nominal th… さらに表示

Primitive streak (PS) cells were derived from hPSCs according to a previously published protocol16. In brief, hPSCs (H9 line) were dissociated and plated as single cells at 20,… さらに表示

The fabrication protocol for the Geltrex gel bed was based on a ‘sandwich’ set-up previously developed for generating polyacrylamide gel substrates13,31,32. To prepare a … さらに表示

The elastomeric artificial matrix consisted of a regular PDMS micropost array generated using a microfabrication protocol previously published14. To functionali… さらに表示