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  2. 4% formaldehyde
    Fix the cells in 4% formaldehyde (Catalog #100-1450) for 30 minutes, then wash and resuspend the sample in the recommended buffer before storing the cells in the refrigerator at 2 - 8°C. Long-term cell storage in 4% formaldehyde is not recommended.

    Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage - ST…

    www.stemcell.com/fridge-freeze-or-fix-cells.html
    www.stemcell.com/fridge-freeze-or-fix-cells.html
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    Should I Fix my cells before a cytometry test?
    Fix cells: Depending on the experimental endpoint, you can fix your cells prior to analysis. If you are not planning to use the cells in downstream assays, such as in vitro culture, try fixing the cells to extend the time between purification and analysis by flow cytometry.

    Refrigerate, Freeze, or Fix Cells for Flow Cytometry or Storage - STEMC…

    stemcell.com
    How do I use a fixation and permeabilization kit for flow cytometry?
    Alternatively, you can also use our fixation and permeabilization kit for flow cytometry (ab185917), which is suitable for most sample types. Fix the cells in your chosen fixative. Spin down cells to a pellet (200 g, 5 min, 4°C), discard the supernatant, and resuspend the pellet in fixative. Incubate cells with the fixative as indicated below.

    Flow cytometry protocol | Abcam

    abcam.com
    How do you fix a cell?
    Fixation can be done from 0.5-2%. Prepare your cells for flow cytometry (block, stain, wash etc...) Fix cells on ice for 15-30 minutes on ice, and then wash twice with PBS. It is recommended that data is recorded as soon as possible after staining and fixation is complete, however samples can be left for a few days if needed.

    Fixing Cells with Paraformaldehyde (PFA) for Flow Cytometry

    flowcytometry.utoronto.ca
    Which fixation method is best for flow cytometric analysis?
    A comparative analysis of all these methods showed that fixation using low PFA concentrations (0.5–1%) at 4–8°C for 10 min is the most appropriate fixing method that not only preserves cell structure and shape but also prevents loss of antibody binding for flow cytometric analysis.

    Optimized protocols for isolation, fixation, and flow cytometric

    ncbi.nlm.nih.gov
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  4. utoronto.ca
    https://www.flowcytometry.utoronto.ca › ... › FixingCe… · PDF-bestand

    Fixing Cells with Paraformaldehyde (PFA) for Flow Cytometry

     
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  5. STEMCELL Technologies
    https://www.stemcell.com › fridge-freez…

    Refrigerate, Freeze, or Fix Cells for Flow Cytometry or …

    WEBFix cells: Depending on the experimental endpoint, you can fix your cells prior to analysis. If you are not planning to use the cells in downstream assays, such as in vitro culture, try fixing the cells to extend the time …

  7. Abcam
    https://www.abcam.com › en-us › technical-resources › protocols › fl…

    Flow cytometry protocol - Abcam

  8. Cell Signaling Technology
    https://www.cellsignal.com › ... › flow-cytometry-fix-permeabilize-cells

    How to Fix and Permeabilize Cells for Flow Cytometry

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