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  1. The term ‘zinc finger’ refers to a group of proteins that have structural features folded around a central Zn2+ that binds to cysteine and histidine polypeptide residues, resulting in a finger-like domain that interdigitates into the major groove of the unfolded, candidate DNA helix.

    Zinc Finger - an overview | ScienceDirect Topics

    www.sciencedirect.com/topics/neuroscience/zinc-finger
    www.sciencedirect.com/topics/neuroscience/zinc-finger
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    How does a zinc finger nuclease bind to DNA?
    Schematic diagram of a zinc finger nuclease (ZFN) dimer bound to target DNA. ZF arrays are fused to the FokI nuclease domain to make a custom nuclease that can recognize unique left and right half-sites. The two ZFNs must bind in an inverted tail-to-tail orientation with their C-termini facing each other.

    Engineering and Application of Zinc Finger Proteins and

    ncbi.nlm.nih.gov
    What are zinc finger nucleases?
    Zinc finger nucleases (ZFN) are DNA-binding proteins that allow targeted editing of the genome by creating double-strand breaks in particular genes (Miller et al., 2007 ). In ZFN-mediated mutagenesis, mRNAs encoding two ZFN are injected into zebrafish embryos at the single-cell stage.

    Zinc Finger Nuclease - an overview | ScienceDirect Topics

    sciencedirect.com
    Can zinc-finger proteins be used for DNA recognition?
    The modular structure of zinc-finger proteins has made them an attractive framework for the design of custom DNA-binding proteins. Key to the application of zinc-finger proteins for specific DNA recognition was the development of unnatural arrays that contain more than three zinc-finger domains.

    ZFN, TALEN and CRISPR/Cas-based methods for genome engineering

    ncbi.nlm.nih.gov
    What is a zinc finger protein?
    (a) (Top) Designed zinc-finger protein in complex with target DNA (grey) (PDB ID: 2I13). Each zinc-finger consists of approximately 30 amino acids in an ββα arrangement (inset). Surface residues (−1, 2, 3 and 6) that contact DNA are shown as sticks. Each zinc-finger domain contacts 3–4 base pairs (bps) in the major groove of DNA.

    ZFN, TALEN and CRISPR/Cas-based methods for genome engineering

    ncbi.nlm.nih.gov
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  3. Studies of Zinc Finger Recognize DNA Sequence
    National Center for Biotechnology Information
    Engineering and Application of Zinc Finger Proteins and TALEs for Biomedical Research
    Engineered DNA-binding domains provide a powerful technology for numerous biomedical studies due to their ability to recognize specific DNA sequences. Zinc fingers (ZF) are one of the most common DNA-binding domains and have been extensively studied for a variety of applications, such as gene regula...
    Author: Moon-Soo Kim, …
    Published: 2017/08/08
    Publication: Mol Cells. 2…
    National Center for Biotechnology Information
    Genome Engineering With Zinc-Finger Nucleases
    Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular DNA repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. This ar...
    Author: Dana Carroll
    Published: 2011/08
    Publication: Genetics. 2…
    National Center for Biotechnology Information
    ZFN, TALEN and CRISPR/Cas-based methods for genome engineering
    Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a non-specifi...
    Author: Thomas Gaj, Ch…
    Published: 2013/07
    DOI: 10.1016/j.tibtech.2…
    PubMed
    DNA recognition by Cys2His2 zinc finger proteins
    Cys2His2 zinc fingers are one of the most common DNA-binding motifs found in eukaryotic transcription factors. These proteins typically contain several fingers that make tandem contacts along the DNA. Each finger has a conserved beta beta alpha structure, and amino acids on the surface of the alpha-...
    Author: S A Wolfe
    Published: 2000
    Keywords: National Insti…
     
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    Wikipedia
    https://en.wikipedia.org › wiki › Zinc_finger

    Zinc finger - Wikipedia

    The most common modular assembly process involves combining separate zinc fingers that can each recognize a 3-basepair DNA sequence to generate 3-finger, 4-, 5-, or 6-finger arrays that recognize target sites ranging from 9 basepairs to 18 basepairs in length. See more

    Overview

    A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions (Zn ) which stabilizes the fold. It was originally coined to describe the finger-like appearance of a … See more

    History

    Zinc fingers were first identified in a study of transcription in the African clawed frog, Xenopus laevis in the laboratory of Aaron Klug. A study of the transcription of a particular RNA … See more

    Classes

    Initially, the term zinc finger was used solely to describe DNA-binding motif found in Xenopus laevis; however, it is now used to refer to any number of structures related by their … See more

    See also

    • Biology portal
    • Technology portal
    • B-box zinc finger
    • DNA-binding protein
    • FPG IleRS zinc finger
    • Krüppel associated box See more

    Overview image
    Domain

    Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their … See more

    Applications

    Various protein engineering techniques can be used to alter the DNA-binding specificity of zinc fingers and tandem repeats of such … See more

    Examples

    This entry represents the CysCysHisCys (C2HC) type zinc finger domain found in eukaryotes. Proteins containing these domains include:
    • MYST family histone acetyltransferases
    • Myelin transcription factor Myt1 See more

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  5. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov › pmc › articles

    Engineering and Application of Zinc Finger Proteins and TALEs for ...

  6. ScienceDirect
    https://www.sciencedirect.com › topics › neuroscience › zinc-finger-motif

    Zinc Finger Motif - an overview | ScienceDirect Topics

  7. ScienceDirect
    https://www.sciencedirect.com › topics › neuroscience › zinc-finger-nuc…

    Zinc Finger Nuclease - an overview | ScienceDirect Topics

  8. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov › pmc › articles

    Genome Engineering With Zinc-Finger Nucleases - PMC

  9. ScienceDirect
    https://www.sciencedirect.com › science › article › pii

    Updated understanding of the protein–DNA recognition

  10. Nature
    https://www.nature.com › articles

    Diversifying the structure of zinc finger nucleases for …

    WEBMar 8, 2019 · Here, to enable such high-precision targeting with zinc-finger nucleases (ZFNs), we have developed an expanded set of architectures that collectively increase the configurational options...

  11. Springer
    https://link.springer.com › referenceworkentry

    Zinc Finger Folds and Functions - SpringerLink

  12. Wikipedia
    https://en.wikipedia.org › wiki › Zinc-finger_nuclease

    Zinc-finger nuclease - Wikipedia

  13. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov › pmc › articles

    ZFN, TALEN and CRISPR/Cas-based methods for genome …

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